Hōʻike kā mākou haʻawina e hōʻike ana ʻo Ganoderma lucidum / reishi / lingzhi i nā waiwai antitumor ma nā cell osteosarcoma in vitro.Ua ʻike ʻia ʻo Ganoderma lucidum ke kāohi nei i ka ulu ʻana o ka maʻi kanesa o ka umauma a me ka neʻe ʻana ma ke kāohi ʻana i ka hōʻailona Wnt/β-catenin.Hoʻopaʻa ia i ka maʻi kanesa ma muli o ka hoʻopau ʻana i nā adhesions focal a me ka hoʻokomo ʻana o ka MDM2-mediated Slug degradation.Kāohi ʻo Ganoderma lucidum i ka maʻi kanesa o ka umauma ma o ka hoʻohaʻahaʻa ʻana i ke ala PI3K/AKT/mTOR.
ʻO ka CCK-8 a me ka hoʻokumu ʻana o ka colony assays, no ka loiloi ʻana i ka hopena o Ganoderma lucidum ma ka osteosarcoma cell line viability and proliferation, ua hōʻike ʻo Ganoderma lucidum e kāohi i ka hoʻonui ʻana o nā pūnaewele MG63 a me U2-OS i ka manawa a me ka hilinaʻi ʻana, a hoʻemi i ka hiki i nā pūnae ke kolo.
Hoʻoponopono ʻo Ganoderma lucidum i ka hōʻike ʻana o nā genes proapoptotic, a ua hōʻike ʻia ka nānā ʻana o ka cytometry kahe e hoʻonui ʻia ka apoptosis o nā pūnaewele MG63 a me U2-OS ma hope o ka mālama ʻana me Ganoderma lucidum.ʻO ka neʻe ʻana o ke kelepona ke kumu o nā ʻano ʻano olaola like ʻole, me ka angiogenesis, ka ho'ōla ʻeha, ka mumū, a me ka metastasis maʻi kanesa.Hoʻemi ʻo Ganoderma lucidum i ka neʻe ʻana a me ka hoʻouka ʻana o nā laina kelepona ʻelua a kāohi i ka hoʻonui, neʻe ʻana, a me ka hoʻouka ʻana, a hoʻoulu i ka apoptosis o nā cell osteosarcoma.
ʻO ka hōʻailona Aberrant Wnt / β-catenin pili pili loa i ka hoʻokumu ʻana, metastasis, a me ka apoptosis o nā ʻano maʻi maʻi he nui, me ka hoʻoponopono ʻana o ka hōʻailona Wnt / β-catenin e nānā ʻia i ka osteosarcoma.
I loko o kēia haʻawina, ua hōʻike ka lua-luciferase reporter assays i ka Ganoderma lucidum lapaʻau poloka CHIR-99021-hoʻā Wnt/β-catenin hōʻailona.Ua hōʻoia hou ʻia kēia e kā mākou hōʻike ʻana i ka transcription o Wnt target genes, e like me LRP5, β-catenin, cyclin D1, a me MMP-9, ke kāohi ʻia ke mālama ʻia nā cell osteosarcoma me Ganoderma lucidum.
Ua hōʻike mua nā haʻawina i loko o nā hōʻailona lapaʻau e hoʻonui ʻia ka LRP5 i ka osteosarcoma e pili ana i ka ʻiʻo maʻamau, a ʻo ka ʻōlelo ʻana o LRP5 e hoʻopili ʻia me ka maʻi metastatic a me ke ola ʻole o ka maʻi maʻi ʻole, e hana ana i ka LRP5 i ka hopena therapeutic kūpono no ka osteosarcoma.
ʻO ka β-catenin pono'ī kahi pahuhopu nui ma ke ala hōʻailona Wnt / β-catenin, a ua hoʻonui nuiʻia ka'ōlelo o ka β-catenin i ka osteosarcoma.Ke unuhi ʻia ka β-catenin i loko o ka nucleus mai ka cytoplasm, hoʻāla ʻo ia i ka hōʻike ʻana o kāna mau genes target downstream, ʻo ia hoʻi ka cyclin D1, C-Myc, a me MMPs.
ʻO Myc kekahi o nā proto-oncogenes nui a he hana koʻikoʻi i ka hoʻoponopono ʻana i ka hoʻāla ʻana, transcription, a me ka pale ʻana i ka hōʻike gene. osteosarcoma.
ʻO Cyclin D1 kahi mea hoʻoponopono koʻikoʻi cell cycle G1 phase a hoʻolalelale i ka hoʻololi ʻana o ka pae G1/S.Hiki ke hoʻopōkole i ka pōʻaiapili cell a hoʻoikaika i ka hoʻonui wikiwiki ʻana o ke kelepona i nā ʻano ʻano tumora nui.
ʻO MMP-2 a me MMP-9 nā stromelysins i hiki ke hoʻohaʻahaʻa i nā ʻāpana matrix extracellular, kahi hiʻohiʻona koʻikoʻi no ka angiogenesis tumor a me ka hoʻouka ʻana.
Hōʻike kēia i nā genes target Wnt/β-catenin i ke kuleana koʻikoʻi i ka holomua o ka osteosarcoma, a ʻo ka pale ʻana i kēia mau nodes hōʻailona hiki ke loaʻa kahi hopena therapeutic koʻikoʻi.
Ma hope mai, ua ʻike mākou i ka hōʻike ʻana o ka mRNA a me ka protein o Wnt/β-catenin hōʻailona pili i nā genes i pili i ka PCR a me ke komohana blotting.Ma nā laina kelepona ʻelua, ua kāohi ʻo Ganoderma lucidum i ka hōʻike ʻana o kēia mau protein a me nā genes.Hōʻike hou kēia mau hopena i ka Ganoderma lucidum ke kāohi i ka hōʻailona Wnt/β-catenin ma ka huli ʻana iā LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, a me MMP-9.
ʻO ka E-cadherin he glycoprotein transmembrane i hōʻike nui ʻia i loko o nā pūnaewele epithelial a hoʻopili i ka hoʻopili ʻana ma waena o nā pūnaewele epithelial a me nā pūnaewele stromal.ʻO ka holoi ʻana a i ʻole ka nalo ʻana o ka ʻōlelo E-cadherin e alakaʻi i ka nalowale a nāwaliwali paha o ka pili ʻana ma waena o nā pūnaewele tumo, hiki ke neʻe maʻalahi nā cell tumor, a laila e hoʻoheheʻe ʻia ka maʻi koko, hoʻopuehu, a me ka metastasize.Ma kēia haʻawina, ua ʻike mākou hiki iā Ganoderma lucidum ke hoʻoponopono i ka E-cadherin, a laila e kūʻē i ka Wnt / β-catenin-mediated phenotype o nā cell osteosarcoma.
I ka hopena, hōʻike kā mākou hopena i ka Ganoderma lucidum poloka i ka osteosarcoma Wnt / β-catenin hōʻailona a alakaʻi i ka emi ʻana o ka hana cell osteosarcoma.Hōʻike kēia mau ʻike e lilo paha ʻo Ganoderma lucidum i mea lapaʻau maikaʻi a maikaʻi hoʻi no ka mālama ʻana i ka osteosarcoma, nā huahana pili pū kekahi.ganoderma lucidum spore oil softgels/reishi spore oil nā ʻeleʻele palupalu,ganoderma lucidum spore pauda/reishi spore pauda
Ka manawa hoʻouna: Apr-18-2022